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Toronto, March 20 Amid the novel coronavirus pandemic crisis, Canadian researchers will use a pioneering imaging technology called Mango, for its bright colour to develop coronavirus testing kits, says a new study.According to the findings, published in the journal Nature Communications, researchers at Simon Fraser University in Canada, developed Mango to sensitively detect RNA molecules, helping to improve viral screening for viruses such as the coronavirus while enabling basic discoveries into the functioning of cells.The latest study by researchers, Peter Unrau and Lena Dolgosheina, involves using Mango to detect individual molecules of RNA within a living cell."We are made of molecules so when something goes wrong within a cell it happens at the molecular level, said study lead author Peter Unrau from Simon Fraser University in Canada."We are using the Mango system as a catalyst, to allow us to not only extend fundamental research questions but also to detect pathogens like the coronavirus, faster and more efficiently," Unrau added.According to the researchers, the Mango system consists of an RNA Mango aptamer that binds tightly and specifically to a fluorescent dye. The aptamer acts like a magnet - targeting and binding those dye molecules.The dye becomes excitable when bound and glows brightly. RNA molecules modified to contain the aptamer 'magnet' now stand out from the other parts of the cell, which makes it much easier for researchers to see and study RNA molecules under a microscope, they said."Cell regulation takes place at the level of RNA. For a long time, the focus has been on protein but it is RNA and not protein that regulates the vast majority of processes within a cell," Unrau said.RNA Mango dyes are currently available from Applied Biological Materials (ABM) in Richmond, B.C.The coronavirus research made possible by Canadian Institutes of Health Research (CIHR) funding will allow the team to develop an isothermal testing methodology, known as Mango NASBA (nucleic acid sequence-based amplification).The Mango NASBA kits can be used to test for the coronavirus, which is a positive strand RNA virus. ABM is actively involved with this project as a partner and will supply the enzymes and buffers needed, which the SFU team originally developed."Mango technology is state of the art and the development of effective cures for cancer and other diseases demand better imaging methodologies to rapidly learn how cells work in detail," Unrau added.( With inputs from IANS )

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